WebDec 1, 1999 · A highly sensitive method for determination of insulin receptor (IR) kinase activity in whole cells, which is based on a STAT5 (signal transducer and activator of transcription 5)-dependent reporter gene assay, has been developed. We show in Rat1 fibroblasts stably overexpressing the human IR (Rat1- …
CLSI C60: Assay Validation & Post-Validation Monitoring - AACC
WebSTAT5 reporter system is designed to monitor the STAT5 transcriptional activity. STAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling pathways. Assay results will contain a written report and downloadable Excel- or Prism-based data. WebThe STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response … The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive … Recombinant Sortase A, His-tag (BPS Bioscience #71086) dose-dependent … thetrainline customer service
STAT5 - an overview ScienceDirect Topics
WebHygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line Choose a response element ISRE SBE SIE STAT5 RE Size 20µg Catalog number selected: E4041 $ 435.00 Your price: Log in Add to Cart Add to Helix Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors … Webperforming dual-luciferase reporter assays. 3) Firefly Luciferase (Fluc) Lentivirus (BPS Bioscience, #79692-G, #79692-H, #79692-P): ... STAT5 Reporter (Luc)-BaF3 cell line 79772 2 vials ONE-Step™ Luciferase Assay System 60690-1 10 ml ONE-Step™ Luciferase Assay System 60690-2 100 ml ... WebApr 1, 2011 · The STAT5 reporter assays in a 96-well plate format. The 32D/IL-2Rβ/6xSTAT5 reporter cells were deprived of WEHI-3 cell-conditioned medium for 6 h. The cells were then resuspended in the absence of WEHI-3 cell-conditioned medium (4 × 10 5 cells/mL) and treated with IL-2 (20 ng/mL) or IL-3 (1 ng/mL) to activate JAK3 or JAK2, respectively. severely itchy legs