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Stat5 reporter assay

WebDec 1, 1999 · A highly sensitive method for determination of insulin receptor (IR) kinase activity in whole cells, which is based on a STAT5 (signal transducer and activator of transcription 5)-dependent reporter gene assay, has been developed. We show in Rat1 fibroblasts stably overexpressing the human IR (Rat1- …

CLSI C60: Assay Validation & Post-Validation Monitoring - AACC

WebSTAT5 reporter system is designed to monitor the STAT5 transcriptional activity. STAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling pathways. Assay results will contain a written report and downloadable Excel- or Prism-based data. WebThe STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response … The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive … Recombinant Sortase A, His-tag (BPS Bioscience #71086) dose-dependent … thetrainline customer service https://consival.com

STAT5 - an overview ScienceDirect Topics

WebHygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line Choose a response element ISRE SBE SIE STAT5 RE Size 20µg Catalog number selected: E4041 $ 435.00 Your price: Log in Add to Cart Add to Helix Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors … Webperforming dual-luciferase reporter assays. 3) Firefly Luciferase (Fluc) Lentivirus (BPS Bioscience, #79692-G, #79692-H, #79692-P): ... STAT5 Reporter (Luc)-BaF3 cell line 79772 2 vials ONE-Step™ Luciferase Assay System 60690-1 10 ml ONE-Step™ Luciferase Assay System 60690-2 100 ml ... WebApr 1, 2011 · The STAT5 reporter assays in a 96-well plate format. The 32D/IL-2Rβ/6xSTAT5 reporter cells were deprived of WEHI-3 cell-conditioned medium for 6 h. The cells were then resuspended in the absence of WEHI-3 cell-conditioned medium (4 × 10 5 cells/mL) and treated with IL-2 (20 ng/mL) or IL-3 (1 ng/mL) to activate JAK3 or JAK2, respectively. severely itchy legs

STAT5 Reporter (Luc)- U937 Cell Line (GM-CSF) - BPS Bioscience

Category:Cellular LanthaScreen and beta-lactamase reporter assays for …

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Stat5 reporter assay

STAT5 Reporter Assay Compound Screening Services - Abeomics

WebReadily assessable STAT5-inducible SEAP reporter activity The stability for 20 passages has been verified Functionally tested and guaranteed mycoplasma-free Applications of HEK-Blue™ IL-9 cells: Detection of human IL-9 (100 pg/ml - 100 ng/ml) and murine IL-9 (30 pg/ml -100 ng/ml) Screening of anti-IL-9 and anti-IL-9R antibodies References: 1. WebWe established and validated a rapid, sensitive, flow cytometric based pSTAT5A assay to detect T cell proliferation. We showed that there was a strong correlation between the early CD3/CD28 or polyclonal mitogen …

Stat5 reporter assay

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WebSTAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling … WebReadily assessable STAT5-inducible SEAP reporter activity The stability for 20 passages has been verified Functionally tested and guaranteed mycoplasma-free Applications of HEK …

WebLike the Kinase receptor activation (KIRA) assay, developed by Sadick et al. [1], the STAT5 phosphorylation assay was performed using two separate microtiter plates: the first one … WebMay 12, 2010 · A skewed ratio of pro-inflammatory to anti-inflammatory cytokines, elevated growth factor synthesis and T- and B-lymphocyte activation are 3 hallmarks of rheumatoid arthritis (RA) pathology. Interleukin-6 (IL-6), IL-7, IL-17, IL-12/IL-23 and growth factors, granulocyte macrophage-colony stimulating factor, IL-3, and erythropoietin activate the …

WebFeb 24, 2024 · General strategy for the design of the reporter cell line: [a] signal through most of the immunologically relevant receptors results in the activation of NF-κB 23, 30, … WebThe STAT5 Reporter (Luc)-U937 cell line is designed for monitoring STAT5 signal transduction pathway in the U937 cell line. It contains a firefly luciferase gene driven by …

WebTo analyze STAT5A phosphorylation: based on the following gating strategy (1) forward scatter (FSC) vs. side scatter (SSC) and (2) CD3 vs. SSC, the T cells (CD3 +) were separated ( Supplementary Figure 1 ). Now, after clear …

WebFeb 2, 2024 · STAT5 is a key member of the JAK/STAT core cancer pathway, activated by a plethora of cytokines and growth factors to regulate a wide spectrum of physiologic … severely lackingWebReporter Vector primer 4 (RVprimer4) binding region 4171–4190 Sequence information for the pGL4 Vectors is available online at: www.promega.com/vectors/ Example Protocol In … severely la giWebMar 11, 2024 · There is evidence from cyclin D1-luciferase reporter assays that STAT5 binds at -481 bp of the cyclin D1 promoter . Oct-1 has also been shown to have a role in the transcriptional regulation of cyclin D1. Oct-1 (Octamer-Binding Protein 1) is a transcription factor ubiquitously expressed in the nucleus that contains a POU ... severely knotted hairWebMoreover, bioinformatics analyses and luciferase reporter assay indicated that exosomal miR-182-5p was bound to PPARG 3' UTR sequence and inhibited the expression of PPARG. Collectively, our findings provided a new role of EPCs-EXO in the clinical treatment of diabetic skin wounds. ... (STAT5) to control EPC cell-cycle progression 44. In ... severely limited germany\u0027sWebJan 27, 2003 · As Dex was used in the STAT5 reporter assay to enhance response , we examined whether the effect of E 2 depended on the presence of glucocorticoid. GH-induced STAT5 activity was significantly lower in the absence than the presence of 250 nM Dex (9.1 ± 0.6-fold and 19.6 ± 1.0-fold, respectively; P 2 without or with Dex (55 ± 2% and 58 ± 1% ... severely itchy scalp with no dandruffWebThe following assays are designed for 96-well format. To perform the assay in a different format, the cell number and reagent volume should be scaled appropriately. A. Dose response of STAT5 reporter (Luc) -U937 cells to human GM-CSF 1. Harvest STAT5 reporter (Luc)-U937 cells from culture in the Growth Medium 8A and seed cells at a severely kyphoticWebNov 17, 2016 · Here, we aimed to delineate adipocyte STAT5 functions in the context of lipid metabolism in white adipose tissue. We generated an adipocyte specific knockout of … severely itchy scalp