WebJan 27, 2010 · For brn3a, we used the full length of the brn3a cDNA as a template for synthesis of cRNA probe ( Aizawa et al., 2005 ). To detect mRNA in the adult mouse and rat tissues, the animals were deeply … WebThese analyses reveal that postmitotic neurons arise from the ventricular surface, migrate through a proliferative zone and differentiate in the upper half of the dorsal midbrain. In later stages of development, we propose to use active JNK (phosphorylated JNK) as a marker for clarification of layers during late embryonic stages.
Wnt/β-catenin signaling in midbrain dopaminergic neuron specification ...
WebApr 26, 2013 · Primary antibodies in this study were: anti-Brn3a antibody (1:1,000; [ 23 ]), anti-β-galactosidase (β-Gal; 1:20; #40-1a; Developmental Hybridoma Study Bank (DHSB), Iowa City, IA, USA), anti-Foxa2 (1:500; #3143; Cell Signaling Technology, Danvers, MA, USA), anti-5-hydroxytryptamine (anti-5-HT; 1:500; #20080; ImmunoStar Inc., Hudson, … WebBrain endogenous liver X receptor ligands selectively promote midbrain neurogenesis. Liver X receptors (Lxrα and Lxrβ) are ligand-dependent nuclear receptors critical for ventral … chinese short film animation list youtube
Foxa1 and Foxa2 regulate multiple phases of midbrain …
WebMay 1, 2024 · Somatic cells have previously been reprogrammed by transcription factors (TFs) into sensory ganglion (SG) neurons but not SG organoids. We identify a combination of triple TFs Ascl1, Brn3b/3a, and... WebNov 28, 2013 · In β-catenin loss-of-function experiments, the midbrain FP resembles the hindbrain FP, and key mDA progenitor genes such as Otx2, Lmx1a, Msx1, and Ngn2 are downregulated whereas Shh is maintained. Accordingly, the neurogenic capacity of the midbrain FP is diminished, resulting in fewer mDA neurons. WebMay 14, 2024 · The Brn3a:GFP expression labels the dHbM, the terminals of these nuclei innervate the ventral IPN primarily. In the adult there is also an intermediate zone of the IPN where in the Tg (narp:GAL4VP16; … chinese shoreham by sea